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Contents of README:PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design. PerlPrimer's current features include the following: - Calculation of possible primer-dimers - Retrieval of genomic or cDNA sequences from Ensembl (including both sequences automatically for QPCR) - Ability to BLAST search primers using the NCBI server or a local server - Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms - Ability to add cloning sequences to primers, automatically adjusted to be in-frame - QPCR primer design without manual intron-exon boundary entry For details and citation: Marshall OJ. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 2004 20(15):2471-2472 For Real Time PCR, spidey is required. |
Name Last modified Size
Parent Directory - README 29-Sep-2012 06:34 1.0K References 05-Sep-2011 03:34 158 doinst.sh 05-Sep-2011 03:34 129 perlprimer.SlackBuild 18-Dec-2011 00:00 2.4K perlprimer.desktop 05-Sep-2011 03:34 130 perlprimer.info 29-Sep-2012 06:34 328 perlprimer.png 05-Sep-2011 00:00 4.8K slack-desc 18-Dec-2011 03:37 958
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